Aspartate transaminase (AST)


Horder M and Bowers GN, 1977, Clin Chem, 23, 551-4 , Biological variability in aspartate aminotransferase activity in serum of healthy persons, and effect of in vitro supplementation with pyridoxal 5-phosphate


Total Score


Parameter Score
1. Scale A
2. Study Population A
3. Samples A
4. Measurand C
5. Pre-Analytical A
6. Replicate Analysis A
7. Steady State B
8. Outlier Analysis C
9. Normality B
10. Variance Homogeneity C
12. CI A
13. No of Results A
14. Concentrations A
15. Total Score C4,8,10
Comment * obsolete method (IFCC at 30°C)

Item Details

Parameter Score
Matrix Serum
Measurand Aspartate transaminase (AST)
Unit U / L
Overall Analytical Principle Method Enzymatic analysis - Not specified
Number of Subjects 6
Number of Subjects included in BV estimation 6
Ethinicity Caucasian
Age Mean / Median
Age Min 24.0
Age Max 46.0
Age SD
Number of Males
Number of Females 6
State of Well Being
Healthy (excl pregnant)
Study Duration Length 15
Study Duration Units Day
Sampling Intervals, e.g. X sample(s) per Interval Unit 4
Interval Units Week
Sampling Times Start Time: 09:00
End Time: 09:00
Number of Samples 8
Average Number of Samples Used for BV Estimation 8.0
Average Number of Replicates 2.0
Estimates of CVi 5.28
Calculated Confidence Intervals of CVi Lower: 4.04, Upper: 7.01
Estimates of CVg 13.58
Calculated Confidence Intervals of CVg Lower: 8.60, Upper: 30.18
Analytical CV 3.61
Calculated Confidence Intervals of Analytical CV Lower: 3.01, Upper: 4.51
Measurand Mean 9.9
Measurand Min
Measurand Max
Measurand SD