Reference
Cavalier E, Lukas P, Bottani M, Aarsand A.K., Ceriotti F, Coşkun A, Diaz-Garzón J, Fernàndez-Calle P, Guerra E, 10 & M. Locatelli, Sandberg S, Carobene A, & on behalf of the European Federation of Clinical Chemistry and Laboratory Medicine Working Group on Biological Variation and IOF-IFCC Committee on Bone Metabolism, 2020, Osteoporos Int, 31(8), 1461-1470 , European Biological Variation Study (EuBIVAS): within- and between-subject biological variation estimates of β-isomerized C-terminal telopeptide of type I collagen (β-CTX), N-terminal propeptide of type I collagen (PINP), osteocalcin, intact fibroblast growth factor 23 and uncarboxylated-unphosphorylated matrix-Gla protein—a cooperation between the EFLM Working Group on Biological Variation and the International Osteoporosis Foundation-International Federation of Clinical Chemistry Committee on Bone Metabolism
| Parameter | Score |
|---|---|
| 1. Scale | A |
| 2. Study Population | A |
| 3. Samples | A |
| 4. Measurand | A |
| 5. Pre-Analytical | A |
| 6. Replicate Analysis | A |
| 7. Steady State | A |
| 8. Outlier Analysis | A |
| 9. Normality | A |
| 10. Variance Homogeneity | A |
| 11. ANOVA | A |
| 12. CI | A |
| 13. No of Results | A |
| 14. Concentrations | A |
| 15. Total Score | A1,2,3,4,5,6,7,8,9,10,11,12,13,14 |
| Comment |
| Parameter | Score |
|---|---|
| Matrix | Serum |
| Measurand | Uncarboxylated-unphosphorylated matrix-Gla protein (uCuP-MGP) |
| Unit | pmol / L |
| Overall Analytical Principle Method | Chemiluminescence |
| Number of Subjects | 91 |
| Number of Subjects included in BV estimation | 85 |
| Ethinicity | Caucasian |
| Age Mean / Median | |
| Age Min | 21.0 |
| Age Max | 69.0 |
| Age SD | |
| Number of Males | 38 |
| Number of Females | 53 |
| State of Well Being |
Healthy (excl pregnant)
|
| Diseases | |
| Study Duration Length | 10 |
| Study Duration Units | Week |
| Sampling Intervals, e.g. X sample(s) per Interval Unit | 1 |
| Interval Units | Week |
| Sampling Times | Start Time:
8:00
End Time: 10:00 |
| Number of Samples | 10 |
| Average Number of Samples Used for BV Estimation | 10.0 |
| Average Number of Replicates | 2.0 |
| Estimates of CVi | 6.9 |
| Calculated Confidence Intervals of CVi | Lower: 6.37, Upper: 7.46 |
| Estimates of CVg | 13.9 |
| Calculated Confidence Intervals of CVg | Lower: 12.02, Upper: 16.44 |
| Analytical CV | 6.9 |
| Calculated Confidence Intervals of Analytical CV | Lower: 6.59, Upper: 7.24 |
| Measurand Mean | 397.0 |
| Measurand Min | 393.0 |
| Measurand Max | 401.0 |
| Measurand SD |