25-hydroxy vitamin D3


Jakobsen J, Bysted A, Andersen R, Bennett T, Brot C, Bügel S, Cashman KD, Denk E, Harrington M, Teucher B, Walczyk T,Ovesen L, 2009, Scand J Clin Lab Invest, 69(2), 190–197 , Vitamin D status assessed by a validated HPLC method: within and between variation in subjects supplemented with vitamin D3


Total Score


Parameter Score
1. Scale A
2. Study Population D
3. Samples NA
4. Measurand NA
5. Pre-Analytical NA
6. Replicate Analysis NA
7. Steady State NA
8. Outlier Analysis NA
9. Normality NA
10. Variance Homogeneity NA
12. CI NA
13. No of Results NA
14. Concentrations NA
15. Total Score D2
Comment no information about health status of subjects is provided

Item Details

Parameter Score
Matrix Serum
Measurand 25-hydroxy vitamin D3
Unit nmol / L
Overall Analytical Principle Method Chromatography; HPLC
Number of Subjects 92
Number of Subjects included in BV estimation 92
Ethinicity Caucasian
Age Mean / Median 62.0
Age Min
Age Max
Age SD
Number of Males 0
Number of Females 92
State of Well Being
Healthy (excl pregnant)
Study Duration Length 20
Study Duration Units Month
Sampling Intervals, e.g. X sample(s) per Interval Unit 16
Interval Units Week
Sampling Times Start Time:
End Time:
Number of Samples 5
Average Number of Samples Used for BV Estimation 5.0
Average Number of Replicates
Estimates of CVi
Calculated Confidence Intervals of CVi Lower: , Upper:
Estimates of CVg
Calculated Confidence Intervals of CVg Lower: , Upper:
Analytical CV
Calculated Confidence Intervals of Analytical CV Lower: , Upper:
Measurand Mean
Measurand Min
Measurand Max
Measurand SD