25-hydroxy vitamin D3

Included in meta analysis


Cavalier E, Fraser CG, Bhattoa HP, Heijboer AC, Makris K , Ulmer CZ , Vesper HW, Vasikaran S, Lukas P, Delanaye P, Carobene A, m, 2021 , Nutrients, 13(2):431, , Analytical Performance Specifications for 25-Hydroxyvitamin D Examinations


Total Score


Parameter Score
1. Scale A
2. Study Population A
3. Samples A
4. Measurand A
5. Pre-Analytical A
6. Replicate Analysis A
7. Steady State A
8. Outlier Analysis A
9. Normality A
10. Variance Homogeneity A
12. CI A
13. No of Results A
14. Concentrations A
15. Total Score A1,2,3,4,5,6,7,8,9,10,11,12,13,14

Item Details

Parameter Score
Matrix Plasma
Measurand 25-hydroxy vitamin D3
Unit ng / mL
Overall Analytical Principle Method Immunoassay; Non-competitive
Number of Subjects 53
Number of Subjects included in BV estimation 32
Ethinicity Caucasian
Age Mean / Median
Age Min
Age Max
Age SD
Number of Males
Number of Females 53
State of Well Being
Healthy (excl pregnant)
Study Duration Length 10
Study Duration Units Week
Sampling Intervals, e.g. X sample(s) per Interval Unit 1
Interval Units Week
Sampling Times Start Time: 8:00 am
End Time: 10:00 pm
Number of Samples 10
Average Number of Samples Used for BV Estimation 9.03
Average Number of Replicates 2.0
Estimates of CVi 6.4
Calculated Confidence Intervals of CVi Lower: 5.88, Upper: 7.02
Estimates of CVg 36.2
Calculated Confidence Intervals of CVg Lower: 29.08, Upper: 47.92
Analytical CV 1.5
Calculated Confidence Intervals of Analytical CV Lower: 1.39, Upper: 1.63
Measurand Mean 23.4
Measurand Min 22.7
Measurand Max 24.2
Measurand SD