25-hydroxy vitamin D3


Lacher DA, Hughes JP and Carroll MD, 2010, National Health Statistics Reports , 21, , Biological variation of laboratory analytes based on the 1999–2002 National Health and Nutrition Examination Survey


Total Score


Parameter Score
1. Scale A
2. Study Population A
3. Samples A
4. Measurand A
5. Pre-Analytical C
6. Replicate Analysis B
7. Steady State C
8. Outlier Analysis A
9. Normality B
10. Variance Homogeneity C
12. CI A
13. No of Results A
14. Concentrations A
15. Total Score C5,7,10

Item Details

Parameter Score
Matrix Serum
Measurand 25-hydroxy vitamin D3
Unit ng / mL
Overall Analytical Principle Method Spectrophotometry; UV/VISIBLE
Number of Subjects 828
Number of Subjects included in BV estimation 460
Ethinicity Hispanic or Latino
Age Mean / Median
Age Min 16.0
Age Max 69.0
Age SD
Number of Males
Number of Females
State of Well Being
Healthy (excl pregnant)
Study Duration Length 36
Study Duration Units Month
Sampling Intervals, e.g. X sample(s) per Interval Unit 1
Interval Units Other
Sampling Times Start Time:
End Time:
Number of Samples 2
Average Number of Samples Used for BV Estimation 2.0
Average Number of Replicates 1.0
Estimates of CVi 21.2
Calculated Confidence Intervals of CVi Lower: 19.64, Upper: 22.89
Estimates of CVg
Calculated Confidence Intervals of CVg Lower: 35.44, Upper:
Analytical CV 8.2
Calculated Confidence Intervals of Analytical CV Lower: 7.17, Upper: 9.58
Measurand Mean 7.802
Measurand Min
Measurand Max
Measurand SD