25-hydroxy vitamin D3

Included in meta analysis


Viljoen A, Singh DK, Farrington K and Twomey PJ, 2011, J Clin Lab Anal, 25, 130-3 , Analytical quality goals for 25-vitamin D based on biological variation


Total Score


Parameter Score
1. Scale A
2. Study Population A
3. Samples A
4. Measurand A
5. Pre-Analytical A
6. Replicate Analysis B
7. Steady State C
8. Outlier Analysis C
9. Normality B
10. Variance Homogeneity C
12. CI C
13. No of Results C
14. Concentrations A
15. Total Score C7,8,10,12,13

Item Details

Parameter Score
Matrix Serum
Measurand 25-hydroxy vitamin D3
Unit nmol / L
Overall Analytical Principle Method Immunoassay; ELISA
Number of Subjects 20
Number of Subjects included in BV estimation 20
Ethinicity Mixed
Age Mean / Median 37.0
Age Min 19.0
Age Max 60.0
Age SD
Number of Males 10
Number of Females 10
State of Well Being
Healthy (excl pregnant)
Study Duration Length 5
Study Duration Units Week
Sampling Intervals, e.g. X sample(s) per Interval Unit 1
Interval Units Week
Sampling Times Start Time: 08:45
End Time: 9:30
Number of Samples 5
Average Number of Samples Used for BV Estimation 5.0
Average Number of Replicates 2.0
Estimates of CVi 12.1
Calculated Confidence Intervals of CVi Lower: 10.18, Upper: 14.63
Estimates of CVg 40.3
Calculated Confidence Intervals of CVg Lower: 30.60, Upper: 58.51
Analytical CV 6.7
Calculated Confidence Intervals of Analytical CV Lower: 5.89, Upper: 7.78
Measurand Mean
Measurand Min
Measurand Max
Measurand SD