25-hydroxy vitamin D3


Lutsey P, Parrinello CM, Misialek JR et al, 2016, Clin Chem, 12, 1647-53 , Short-term variability of vitamin D-related biomarkers


Total Score


Parameter Score
1. Scale A
2. Study Population A
3. Samples A
4. Measurand A
5. Pre-Analytical C
6. Replicate Analysis B
7. Steady State C
8. Outlier Analysis C
9. Normality B
10. Variance Homogeneity C
12. CI A
13. No of Results C
14. Concentrations A
15. Total Score C5,7,8,10,11,13

Item Details

Parameter Score
Matrix Serum
Measurand 25-hydroxy vitamin D3
Unit ng / mL
Overall Analytical Principle Method MS; HPLC-MS
Number of Subjects 160
Number of Subjects included in BV estimation 160
Ethinicity Mixed
Age Mean / Median 76.0
Age Min 68.0
Age Max 88.0
Age SD
Number of Males 64
Number of Females 96
State of Well Being
Non-healthy (excl pregnant)
Study Duration Length 6
Study Duration Units Week
Sampling Intervals, e.g. X sample(s) per Interval Unit 1
Interval Units Other
Sampling Times Start Time:
End Time:
Number of Samples 2
Average Number of Samples Used for BV Estimation 2.0
Average Number of Replicates
Estimates of CVi 6.9
Calculated Confidence Intervals of CVi Lower: 4.95, Upper: 8.30
Estimates of CVg 31.4
Calculated Confidence Intervals of CVg Lower: 28.18, Upper: 35.40
Analytical CV 5.3
Calculated Confidence Intervals of Analytical CV Lower: 4.26, Upper: 7.01
Measurand Mean 34.0
Measurand Min
Measurand Max
Measurand SD 11.1